Projects/science - world/may 26, 2020
About This Project
Low frequency radiation is a very common form of radiation in people’s lives, two examples being cell phones and car radar emitters, used as safety features. Some studies have raised concerns over cell phones, but the available literature remains inconclusive. I am testing E.coli using the Ames test, a test for mutagenic substances, and fruit flies, using both cell phones and radar guns. I hypothesize there will be no change in the rate of mutations among test specimen when exposed.
Some studies have raised safety concerns over low frequency radiation causing items, particularly cell phones. However, the literature seems to prove inconclusive, due to several factors, including the lack of human testing and the reliance of animal trials. Using a variation of the Ames test, from a high school supply website, with E.coli and on fruit flies (Drosophila melanogaster), which both have shorter lifespans, can help serve as a more definitive model for testing whether low frequency radiation items do cause adverse effects. In this experiment, I will be testing using cell phones, and a radar gun to simulate car radar emitters. The Ames test is something which is new to the field, and fruit flies have only been used a limited number of times in these studies.
Cell phones and cars are some of the most common items in our lives. Seeing as there is a possibility that these items have adverse effects on human beings, the public should be informed as quickly as possible, as teenagers in particular spend hours a day on their phones, and many people own and drive at least one car, and if they do not are exposed to low frequency radiation when they are passed by a car. If my data provides support that there are no adverse effects, then there should be no need for safety precautions for using your cell phone, and the opposite is also true, if there are adverse effects, then there should be safety precautions put in place.
The aim of this project is to determine the effects of low-frequency radiation on living organisms. I hope to determine whether or not the cell phone and/or radar gun does cause mutation. The first portion of this study uses a variation of the Ames test only requires biosafety level 1 protocols (due to E. coli). The low-frequency items will be tested and compared to a known mutagen, a control. All of the experimental plates will be placed in a shielded box to eliminate human exposure. A second aim is to determine whether distance from the radiation source changes the effect of low frequency radiation on organisms. If my results demonstrate that cell phones lead to increased mutation rates, limiting exposure to cell phones would be recommended to avoid impacts of radiation.
Distribution of money
To shorten the amount of time to collect data, the well plates would come prepped with the E.coli already in each slot of the well plate. The Incubator and Bio Hazard storage are to ensure that the E.coli in the well plates stay alive, and that they can be disposed of properly.
Since I am comparing responses between a prokaryotic organism and a multi cellular eukaryotic organism, I will need to order fruit flies and a E.coli culture kit.
For this experiment, I will be using my own phone and a radar gun, the latter will simulate the radar emitters in cars. As a positive control, I will be using UV light, which is a known mutagen.
Because E.coli and fruit flies have shorter life spans than many other organisms, trials will be able to progress quickly. The trials using E. Coli plates will have the low-frequency item placed a few inches over the well plates, and each trial will take place for around 3 weeks. The fruit fly trials will also have the low-frequency item placed a few inches over the culture kits, and will take place around 6 weeks, for at least two generations of fruit flies to be tested.
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